Ökophysiologie der Pflanzen

Dr. rer. nat. Frauke Pescheck

Wissenschaftliche Assistentin

Am Botanischen Garten 1-9, R. 306
Telefon: +49 431 880-4291
Telefax: +49 431 880-5568
fpescheck@bot.uni-kiel.de

CV

since 2015

Postdoc Christian Albrechts-University of Kiel, Germany
Ecophysiology of Plants, Botanical Institute
2007-2014

PhD student, Christian Albrechts-University of Kiel, Germany
Supervisor: Professor Dr. W. Bilger, Ecophysiology of Plants, Botanical Institute
2003-2004


Participation in the Master program `Biological Oceanography´ of the University of Kiel
and the South-Danish University (SDU),
stay abroad at the SDU in Odense, Denmark, from February 2002 until July 2002
2002-2006

Main studies of Biology (Dipl.) at the Christian Albrechts-University of Kiel, Germany,
main subject: Marine biology, minor subjects: Botany, Marine chemistry
1999-2002 Basic studies of Biology (Dipl.) at the Leibniz University Hannover, Germany

Research interests

UV-B resistance mechanisms of algae and plants

All photosynthetic organism need to protect themselves against the potentially harmful effects of ultraviolet-B radiation. Major targets of UV-B radiation are the photosynthetic machinery and the DNA. Resistance towards UV-B radiation can either be established by effective shielding or by cellular tolerance mechanisms, like repair of induced damages. One important repair mechanism is the light-dependent splitting of UV-B induced DNA dimers, called photoreactivation. My studies  concentrate on the following:

1. Ulva intestinalis as a model organism of a non-UV screening macroalga:

  • Characterisation of photoreactivation rates under controlled experimental conditions
  • Analysis of the expression of the photolyase gene under laboratory and field conditions combined with physiological activity measurements
  • Investigation of the ultrastructural effects of chloroplasts and nucleus localisation with respect to DNA damage formation and distribution.
  • Searching for other factors which contribute to the DNA stability of Ulva intestinalis by a comparison of the intrinsic sensitivity of the DNA with Arabidopsis thaliana and other plants
Ulva and Cladophora ona stone In Falckenstein

 

2. Cladophora sericea as a model organism for a UV-screening macroalga:

  • Characterisation of the screening substance
  • Determination of the variability of observed screening depending on abiotic factors under field and laboratory conditions

 

Microscopy image of Rhizoclonium riparium Microscopy image of autofluorescence of Rhizoclonium riparium

3. Cross-talk of temperature and solar UV-B radiation on the acclimation of UV-B resistance of Arabidopsis thaliana:

  • Analysis of in vivo UV-screening and pigment composition under field conditions and in controlled environments
  • Regulation of DNA repair by photoreactivation in natural sunlight

 

Quercetin localisation

Quercetin is a dihydroxylated flavonol which has a good UV absorbing and antioxidant capacity. We analysed the occurence of this important compound in Arabidopsis thaliana grown under high light including ultraviolet-B radiation and low air temperature by confocal laser scanning microscopy. Cross-sections were stained with Naturstoff reagent A of Arabidopsis thaliana wildtype (left) and the quercetin deficient tt7 mutant (right). For details see our new article in Physiologia Plantarum (https://doi.org/10.1111/ppl.13554). Yellow fluorescence originates from from quercetin derivatives which can be detected throughout the cross section in the LER wildtype but not in the tt7 mutant. Red chlorophyll fluorescence localises the chloroplasts. adE = adaxial epidermis, abE = abaxial epidermis, Pa = palisade parenchyma, Sp = spongy parenchyma.

Many thanks to Prof. Roeder and Dr. Urska Repnik for the possibility and help to use the Zeiss880!

Many thanks also to Dr. Christine Desel for advice during sample preparation and analysis of the micrographs!

Publications

Pescheck, F., Rosenqvist E., and W. Bilger (2021) Plants and UV-B radiation. Physiologia Plantarum, 173: 762–774https://doi.org/10.1111/ppl.13577

Nichelmann, L. and Pescheck, F. (2021) Solar UV-B effects on composition and UV screening efficiency of foliar phenolics in Arabidopsis thaliana are augmented by temperature. Physiologia Plantarum, 173, 662-674 https://doi.org/10.1111/ppl.13554

Qian, M., Rosenqvist, E., Prinsen, E., Pescheck, F., Flygare, A-M., Kalbina, I., Jansen, M.A.K., and Å. Strid (2021) Downsizing in plants - UV light induces pronounced morphological changes in the absence of stress. Plant Physiology, kiab262, https://academic.oup.com/plphys/advance-article/doi/10.1093/plphys/kiab262/6294311

Pescheck, F. and W. Bilger (2020) UV-B resistance of plants grown under natural conditions. Annual Plant Reviews online, 3, 1-62. doi.org/10.1002/9781119312994.apr0733

Pescheck, F. (2019) UV-A screening in Cladophora sp. lowers internal UV-A availability and photoreactivation as compared to non-UV screening Ulva intestinalis. Photochemical and Photobiological Sciences,18, 413-423. doi:10.1039/C8PP00432C.  http://pubs.rsc.org/en/content/articlehtml/2019/PP/C8PP00432C

Pescheck, F. and W. Bilger (2019) High impact of seasonal temperature changes on acclimation of photoprotection and radiation-induced damage in field grown Arabidopsis thaliana. Plant Physiology and Biochemistry, 134, 129-136.

Pescheck, F. and W. Bilger (2018) Compensation of lack of UV screening by cellular tolerance in green macroalgae (Ulvophyceae) from the upper eulittoral. Marine Biology, 165:132. doi: 10.1007/s00227-018-3393-0 link

Pescheck, F., Campen, H., Nichelmann, L., and W. Bilger (2016) Relative sensitivity of DNA and photosystem II in Ulva intestinalis (Chlorophyta) under natural solar irradiation. Marine Ecology Progress Series, 555, 95-107. https://doi.org/10.3354/meps11802

Pescheck, F., Lohbeck, K., Roleda, M., and W. Bilger (2014) UVB-induced DNA and photosystem II damage in two intertidal green macroalgae: distinct survival strategies in UV-screening and non-screening Chlorophyta. Journal of Photochemistry and Photobiology B: Biology 132, 85–93. doi.org/10.1016/j.jphotobiol.2014.02.006

Pescheck, F., Bischof, K. and W. Bilger (2010) Screening of UV-A and UV-B radiation in marine green macroalgae (Chlorophyta). Journal of Phycology, 46, 444-455. doi.org/10.1111/j.1529-8817.2010.00825.x